Effects of free radicals and amyloid β protein on the currents of expressed rat receptors in Xenopus oocytes

被引：0

作者：

黄福南 ^{[1
]}

李文彬 ^{[1
]}

张炳烈 ^{[1
]}

崔旭 ^{[1
]}

韩志涛 ^{[1
]}

房征宇 ^{[1
]}

蔡竖平 ^{[1
]}

尹岭 ^{[1
]}

王鲁宁 ^{[1
]}

机构：

[1] 解放军总医院老年医学研究所神经生物学与神经疾病实验室!北京100853

来源：

中华医学杂志(英文版) | 2001年 / 03期

关键词：

淀粉样β蛋白·自由基·神经递质·受体·谷氨酸·阿尔茨海默病;

D O I：

暂无

中图分类号：

R321 [人体胚胎学（人体胎生学、发生学）];

学科分类号：

071008 ;

摘要：

目的　探讨淀粉样 β蛋白 (Aβ1 4 0 )和自由基对爪蟾卵母细胞表达的大鼠脑谷氨酸受体功能的影响。方法　采用Promega试剂盒提取 3月龄大鼠脑组织mRNA ,并将 50nl (50ng)的mRNA显微注射到成熟的每个爪蟾卵母细胞进行受体表达。表达的受体激活电流采用双电极电压钳位技术记录。超氧阴离子自由基 (SAFRs)和Aβ1 4 0 等在记录前的 12h、2 4h、96h分别加入孵育液。结果　注射到爪蟾卵母细胞的鼠脑mRNA可以表达出M型ACh、谷氨酸等受体 ,这些受体的激活电流特征是由氯离子载流的内向电流。Aβ1 4 0 对表达的谷氨酸受体有抑制效应 ,其程度依作用时间和浓度不同而不同。 2 0nmol/LAβ1 4 0 作用 2 4小时对表达的谷氨酸受体功能无影响 ,SAFRs共孵育时 2 0nmol/LAβ1 4 0 作用 12小时即对表达的谷氨酸受体功能有明显影响。与SAFRs共同孵育时 6 0nmol/LAβ1 4 0 作用 12小时即对表达的谷氨酸受体有明显抑制效应 (降低 2 1% ,P <0 0 5) ,而作用 2 4小时时下降达 52 %。维生素E对这些效应有拮抗作用。结论　自由基和Aβ对谷氨酸受体具有抑制效应 ,该效应能被维生素E拮抗。Aβ可通过抑制受体功能在阿尔茨海默病发病机理中起作用

引用

页码：20 / 23+103

页数：5

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