A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering

被引：0
|
作者：
Yiman Geng [1 ]
Haiqin Yan [2 ]
Pei Li [1 ]
Gaixian Ren [1 ]
Xiaopeng Guo [1 ]
Peiqi Yin [1 ]
Leiliang Zhang [1 ]
Zhaohui Qian [1 ]
Zhendong Zhao [1 ]
Yi-Cheng Sun [1 ]
机构：
[1] NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Center for Tuberculosis Research,Chinese Academy of Medical Sciences and Peking Union Medical College
[2] Department of Basic Medical Sciences,Anhui Key Laboratory of Infection and Immunity,Bengbu Medical College
来源：
Journal of Genetics and Genomics | 2019年 / 46卷 / 09期
基金：
中国国家自然科学基金;
关键词：
RNA; Red recombineering; A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage; CRISPR;
D O I：
暂无
中图分类号：
Q78 [基因工程（遗传工程）];
学科分类号：
071007 ; 0836 ; 090102 ;
摘要：
Plasmids are useful tools for studying genetic information in living cells,as well as heterologous expression of genes and pathways in cells (Lauritsen et al.,2018).Various methods have been developed for plasmid manipulation both in vivo and in vitro(Aslanidis and de Jong,1990;Li and Elledge,2007;Xia et al.,2018).However,large plasmids,such as P1-based artificial chromosomes (PACs),bacterial artificial chromosomes (BACs),and fosmids,
引用
收藏
页码：452 / 455
页数：4
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