Prediction of Retention Time of Solutes under Linear Gradient Elution Conditions in RP-HPLC

被引:0
|
作者
杨更亮
田宝娟Department of Chemistry
Normal College of Baoding
Baoding
机构
[1] College of Chemistry and Environmental Science, Hebei University,Molecular Science Center,Institute of Chemistry,Chinese Academy of Sciences,College of Chemistry and Environmental Science, Hebei University Baoding, Hebei 071002, China
[2] Molecular Science Center, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China,Hebei 071051, China,Beijing 100080, China,Baoding, Hebei 071002, China
关键词
high performance liquid chromatography; gradient elution; retention time; prediction;
D O I
暂无
中图分类号
O657.7 [色谱分析];
学科分类号
070302 ; 081704 ;
摘要
A model for predicting retention time of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the retention behaviors of p hydroxybenzaldehyde, vanillin, biphenyl, phenanthrene in gradient elution. With taking into account the dwell time of the instrumental system, the theoretically predicted retention times agree well with those experimentally determined.
引用
收藏
页码:1084 / 1087 +980
页数:5
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