Expression of p27kip1, Rb protein and proliferating cell nuclear antigen and its relationship with clinicopathology in human pancreatic cancer

OBJECTIVE: To investigate the effect of inhibiting factor of cell cycle regulation p27,retinoblastinoma protein (Rb protein), and proliferating cell nuclear antigen (PCNA) on the genesis andprogression of human pancreatic cancer.METHODS: The expression of p27, Rb protein and PCNA in the tumor tissue and adjacent tissue of32 patients with pancreatic cancer was detected by SP immunohistochemical technique.RESULTS: The p27protein positive-expression rate in the tumor tissue of pancreatic cancer was56.25%, which was lower than that in the adjacent pancreatic tissue (P<0.05). p27proteinpositive-expression was correlated significantly with tumor cell differentiation and lymph node metastasis(P<0.05). The Rb gene protein positive-expression rate in the tumor tissue was 50%, which was alsolower than that in the adjacent pancreatic tissue (P<0.05 ). The PCNA positive-expression rate was71.87%, which was higher than that in the adjacent pancreatic tissue (P<0.05). PCNA positive-expression was also correlated significantly with tumor cell differentiation and lymph node metastasis(P<0.05).CONCLUSION: The decreased expression of p27, Rb protein and over-expression of PCNA may playan important role in the genesis and progression of pancreatic cancer.