Antifungal Activity of Aspidin BB from Dryopteris fragrans against Trichophyton rubrum Involved Inhibition of Ergosterol Biosynthesis

被引：0
|
作者：
Shi-hao Yang [1 ]
Wen-hao Chen [1 ]
Feng Shan [2 ]
Xiao-zhou Jia [1 ]
Rong-rong Deng [1 ]
Chu-ping Tang [1 ]
Zhi-bin Shen [1 ]
机构：
[1] Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University
[2] Department of Pharmaceutical Science, School of Pharmacy University of Pittsburgh
来源：
Chinese Herbal Medicines | 2017年 / 01期
关键词：
Aspidin BB; Dryopteris fragrans; ergosterol; squalene epoxidase; Trichophyton rubrum;
D O I：
暂无
中图分类号：
R284 [中药化学]; R285 [中药药理学];
学科分类号：
1008 ;
摘要：
Objectives Aspidin BB, a typical phloroglucinol derivative from Dryopteris fragrans, possesses significant antifungal property. This study aimed to investigate potential mechanism of antifungal activity of Aspidin BB against Trichophyton rubrum which is the most common pathogens responsible for chronic dermatophytosis. Methods The minimum inhibitory concentration(MIC) of Aspidin BB against strains was determined by broth microdilution. The effects of Aspidin BB on ergosterol biosynthesis were investigated by content determination based on UPLC method. Besides, the effects of drugs on squalene epoxidase(SE) in T. rubrum cell membrane were analyzed. Results MIC value of Aspidin BB against T. rubrum was 25.0 μg/m L. Aspidin BB reduced ergosterol content significantly, but no notable effect on squalene epoxidase activity. Conclusion The results suggested that Aspidin BB inhibited ergosterol biosynthesis.However, it was not squalene epoxidase but other components may sever as possible targets in ergosterol biosynthesis pathway.
引用
收藏
页码：63 / 68
页数：6
相关论文
共 50 条
  • [41] Antifungal activity of essential oils and their synergy with fluconazole against drug-resistant strains of Aspergillus fumigatus and Trichophyton rubrum
    Khan, Mohd Sajjad Ahmad
    Ahmad, Iqbal
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 90 (03) : 1083 - 1094
  • [42] Antifungal Efficacy of Gallic Acid Extracted From Pomegranate Peel Against Trichophyton rubrum: In Vitro Case Study
    Fan, GaoFu
    Xu, ZhenGuo
    Liu, XiuShu
    Yin, Wei
    Sun, LiHua
    Wu, Dan
    Wei, MengQiang
    Wang, Wei
    Cai, YuHua
    NATURAL PRODUCT COMMUNICATIONS, 2023, 18 (01)
  • [43] Expression, purification and in vitro antifungal activity of acidic mammalian chitinase against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains
    Chen, L.
    Shen, Z.
    Wu, J.
    CLINICAL AND EXPERIMENTAL DERMATOLOGY, 2009, 34 (01) : 55 - 60
  • [44] Isolation and antifungal activity of lignans from Myristica fragrans against various plant pathogenic fungi
    Cho, Jun Young
    Choi, Gyung Ja
    Son, Seung Wan
    Jang, Kyoung Soo
    Lim, He Kyoung
    Lee, Sun Og
    Do Sung, Nack
    Cho, Kwang Yun
    Kim, Jin-Cheol
    PEST MANAGEMENT SCIENCE, 2007, 63 (09) : 935 - 940
  • [45] Chiral N-benzyl-N-methyl-1-(naphthalen-1-yl)ethanamines and their in vitro antifungal activity against Cryptococcus neoformans, Trichophyton mentagrophytes and Trichophyton rubrum
    Thvedt, Thor H. Krane
    Kaasa, Kristin
    Sundby, Eirik
    Charnock, Colin
    Hoff, Bard Helge
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2013, 68 : 482 - 496
  • [46] In Vitro Activity of Antifungal Drugs Against Trichophyton rubrum and Trichophyton mentagrophytes spp. by E-Test Method and Non-supplemented Mueller–Hinton Agar Plates
    Jari Intra
    Cecilia Sarto
    Selene Mazzola
    Chiara Fania
    Natalia Tiberti
    Paolo Brambilla
    Mycopathologia, 2019, 184 : 517 - 523
  • [47] Characterization of squalene epoxidase activity from the dermatophyte Trichophyton rubrum and its inhibition by terbinafine and other antimycotic agents
    Favre, B
    Ryder, NS
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (02) : 443 - 447
  • [48] Antifungal activity of Cardiospermum halicacabum L. (Sapindaceae) against Trichophyton rubrum occurs through molecular interaction with fungal Hsp90
    Gaziano, Roberta
    Campione, Elena
    Iacovelli, Federico
    Marino, Daniele
    Pica, Francesca
    Di Francesco, Paolo
    Aquaro, Stefano
    Menichini, Francesco
    Falconi, Mattia
    Bianchi, Luca
    DRUG DESIGN DEVELOPMENT AND THERAPY, 2018, 12 : 2185 - 2193
  • [49] P-27530: In vitro evaluation of antifungal activity against a broad spectrum of yeast and filamentous fungi: investigation of the evolution of resistance in Trichophyton rubrum
    Long, L. A.
    Isham, N.
    Stoll, G.
    Gueye, S. A.
    Ghelardi, E.
    Celandroni, F.
    Mailland, F.
    Bulgheroni, A.
    Ghannoum, M. A.
    MYCOSES, 2013, 56 : 57 - 58
  • [50] Evaluation of antifungal activity of glycoalkaloids from the Solanum lycocarpum St. Hil (lobeira) in the cell membrane of dermatophyte of Trichophyton rubrum
    Bruna AM Cantelli
    Flavia R Barbosa
    Tamires A Bitencourt
    Mariza A Miranda
    Jairo K Bastos
    Mozart Marins
    Ana Lucia Fachin
    Mariana H De Abreu
    Yasmin D Crivelenti
    Thaís B Mesquita
    BMC Proceedings, 8 (Suppl 4)
12345