Antifungal Activity of Aspidin BB from Dryopteris fragrans against Trichophyton rubrum Involved Inhibition of Ergosterol Biosynthesis

被引：0
|
作者：
Shi-hao Yang [1 ]
Wen-hao Chen [1 ]
Feng Shan [2 ]
Xiao-zhou Jia [1 ]
Rong-rong Deng [1 ]
Chu-ping Tang [1 ]
Zhi-bin Shen [1 ]
机构：
[1] Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University
[2] Department of Pharmaceutical Science, School of Pharmacy University of Pittsburgh
来源：
Chinese Herbal Medicines | 2017年 / 01期
关键词：
Aspidin BB; Dryopteris fragrans; ergosterol; squalene epoxidase; Trichophyton rubrum;
D O I：
暂无
中图分类号：
R284 [中药化学]; R285 [中药药理学];
学科分类号：
1008 ;
摘要：
Objectives Aspidin BB, a typical phloroglucinol derivative from Dryopteris fragrans, possesses significant antifungal property. This study aimed to investigate potential mechanism of antifungal activity of Aspidin BB against Trichophyton rubrum which is the most common pathogens responsible for chronic dermatophytosis. Methods The minimum inhibitory concentration(MIC) of Aspidin BB against strains was determined by broth microdilution. The effects of Aspidin BB on ergosterol biosynthesis were investigated by content determination based on UPLC method. Besides, the effects of drugs on squalene epoxidase(SE) in T. rubrum cell membrane were analyzed. Results MIC value of Aspidin BB against T. rubrum was 25.0 μg/m L. Aspidin BB reduced ergosterol content significantly, but no notable effect on squalene epoxidase activity. Conclusion The results suggested that Aspidin BB inhibited ergosterol biosynthesis.However, it was not squalene epoxidase but other components may sever as possible targets in ergosterol biosynthesis pathway.
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页码：63 / 68
页数：6
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