Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells in vitro

被引：0
|
作者：
Wen Lu [1 ]
Da Duan [2 ]
Zacharia Ackbarkhan [1 ]
Ming Lu [2 ]
Min-Li Huang [1 ]
机构：
[1] Department of Ophthalmology,the First Affiliated Hospital of Guangxi Medical University
[2] Department of Neurosurgery,the 163rd Hospital of Chinese PLA,the Second Affiliated Hospital of Hunan Normal University
来源：
International Journal of Ophthalmology | 2017年 / 10卷 / 10期
关键词：
human olfactory mucosa; mesenchymal stem cells; differentiation; retinal cells;
D O I：
暂无
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
AIM:To investigate whether the human olfactory mucosa mesenchymal stem cells(OM-MSCs) can differentiate into photoreceptor cells in vitro.METHODS:Through the olfactory mucosa adherent method,olfactory mucosa was isolated,cultured and identified in vitro among mesenchymal stem cells.The cell surface markers were analyzed by flow cytometry,induced to differentiate into retinal photoreceptor cells in vitro,and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods.RESULTS:OM-MSCs from human were spindle cellbased,and showing radial colony arrangement.OM-MSCs were negative for CD34,CD45 and CD105,but positive for CD73 and CD90.Following induction,a strong positive reaction was produced by photoreceptor specific marker rhodopsin in the cells.CONSLUSION:This novel finding demonstrates that OM-MSCs can be cultured and expanded in vitro.They possess biological characteristics of mesenchymal stem cells,and have the ability to be induced into retinal cells.
引用
收藏
页码：1504 / 1509
页数：6
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