Comparison of rabbit corporal smooth muscle cell-culture Methods in vitro

Objective: To apply series or convenient and effective methods to produce large amount of pure corporal smooth muscle cells in vitro in terms of experimental requirement. Methods: The explant cell culture and digestion cell culture methods were compared. Results: The cell yield and purity were significantly higher in digestion cell culture compared with explant cell culture; cell growth velocity was more rapid in digestion cell culture also; but the cell culture success rates were higher in explant cell culture compared with digestion cell culture and the explant cell culture method was much easier to be mastered. Conclusions: Each method had individual merit and shortcoming. The best one of the two methods could be chosen according to experimental requirement.The SMC cultured in vitro are proved to be used to evaluate and investigate the effect of some medicine on penile erection.