Inhibitory　effects　of　nifedipine　on　DNA　and　protein　synthesis　in　cultured　cardiac　nonmyocytes　of　neonatal　rats

被引：0
|
作者：
王洪新 [1 ]
陶亮 [1 ]
饶曼人 [1 ]
机构：
[1] 南京医科大学心血管药理研究室
来源：
ActaPharmacologicaSinica | 1997年 / 02期
关键词：
培养的细胞; 硝苯地平; 血管紧张素Ⅱ; 心脏肥厚; 心脏;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
观察硝苯地平（Ｎｉｆｅｄｉｐｉｎｅ，Ｎｉｆ）对非心肌细胞生长和增殖的抑制作用．方法：以培养乳鼠非心肌细胞作为模型，用［３Ｈ］胸腺嘧啶核苷结合及［３Ｈ］亮氨酸结合的方法．结果：在血管紧张素Ⅱ存在的情况下，Ｎｉｆ１μｍｏｌ·Ｌ－１作用细胞７２ｈ，细胞的总蛋白及细胞数目明显减少．测得血管紧张素Ⅱ１，１０，１００，１０００ｎｍｏｌ·Ｌ－１在４８ｈ内，增加ＤＮＡ合成及蛋白合成．Ｎｉｆ１－１０μｍｏｌ·Ｌ－１均能够减少１００ｎｍｏｌ·Ｌ－１血管紧张素Ⅱ诱导的细胞ＤＮＡ合成及蛋白合成．结论：Ｎｉｆ可以直接抑制非心肌细胞的生长，该种作用与其抑制心肌肥厚有关．
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页码：41 / 44
页数：4
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