Capillary electrophoretic separation of glycoproteins

被引:0
|
作者
陈义
机构
关键词
glycoprotein; microheterogeneity; capillary electrophoresis;
D O I
暂无
中图分类号
O658.9 [其他];
学科分类号
摘要
A capillary electrophoretic approach has been developed for analyzing the microheterogeneity of glycoproteins. The key factors include the buffer composition, concentration, pH, and the surface treatment of the capillaries used. It has been shown that, if the buffer is mainly composed of alcohol amines and the capillaries are coated with polyacrylamide, ovalbumin from chicken egg can be separated into more than 25 peaks and that of transferrin about 10 peaks.
引用
收藏
页码:71 / 76
页数:6
相关论文
共 50 条
  • [41] Electrophoretic separation schemes in ultrathin channels with capillary sample introduction
    Bullard, KM
    Gavin, PF
    Ewing, AG
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1998, 17 (07) : 401 - 410
  • [42] Systematic investigations of different capillary electrophoretic techniques for separation of methylquinolines
    Schöftner, R
    Buchberger, W
    JOURNAL OF SEPARATION SCIENCE, 2003, 26 (14) : 1247 - 1252
  • [43] Capillary zone electrophoretic separation and determination of imidazolic antifungal drugs
    Arranz, A
    Echevarría, C
    Moreda, JM
    Cid, A
    Arranz, JF
    JOURNAL OF CHROMATOGRAPHY A, 2000, 871 (1-2) : 399 - 402
  • [44] Determination of the electrophoretic mobility of bacteria and their separation by capillary zone electrophoresis
    Pfetsch, A
    Welsch, T
    FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1997, 359 (02): : 198 - 201
  • [45] Electrophoretic separation schemes in ultrathin channels with capillary sample introduction
    Department of Chemistry, Pennsylvania State University, 152 Davey Lab, University Park, PA 16802, United States
    TrAC Trends Anal. Chem., 7 (401-410):
  • [46] Separation of flavones by capillary electrophoresis: The influence of pKa on electrophoretic mobility
    Markham, KR
    McGhie, TK
    PHYTOCHEMICAL ANALYSIS, 1996, 7 (06) : 300 - 304
  • [47] Capillary electrophoretic separation of herbicidal enantiomers applying ergot alkaloids
    Ingelse, BA
    Reijenga, JC
    Flieger, M
    Everaerts, FM
    JOURNAL OF CHROMATOGRAPHY A, 1997, 791 (1-2) : 339 - 342
  • [48] On-line Sample Manipulation in Capillary During Electrophoretic Separation
    Tuma, Petr
    Gojda, Jan
    XXXV MODERNI ELEKTROCHEMICKE METODY, 2015, : 255 - 259
  • [49] Capillary electrophoretic separation of metal ions in the presence of polyethylene glycols
    Stathakis, C
    Cassidy, RM
    ANALYST, 1996, 121 (06) : 839 - 843
  • [50] CAPILLARY ELECTROPHORESIS - MANIPULATING THE ELECTROPHORETIC MEDIA TO ENHANCE SEPARATION AND DETECTION
    SEPANIAK, MJ
    SWAILE, DF
    COLE, RO
    POWELL, AC
    SCHONLAWFINNISS, S
    STALLER, TD
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1991, 201 : 135 - ANYL