Research on Antibacterial Effect of Achillea millefolium of Xinjiang

[Objective]To study antibacterial activity of Achillea millefolium extract extracted by different methods,and measure the minimum inhibitory concentration( MIC) and the minimum bactericidal concentration( MBC) of Achillea millefolium extract,which can provide the basis for the development and utilization of Achillea millefolium. [Methods] Effective components were extracted respectively from Achillea millefolium by heat extraction method and cold-maceration method,and Escherichia coli,Penicillium and some pathogenic bacteria were used as tested strains,using Bauer-Kirby method( filter paper method and microscale 2 times continuous gradient dilution test) to conduct vitro antibacterial test in several common strains. [Results]Petroleum ether,85% and 95% ethanol and ethyl acetate extracts of Achillea millefolium have antibacterial effects on Escherichia coli,Staphylococcus aureus,Proteus and Botrytis cinerea. MIC of 85% ethanol extract to the Escherichia coli was 0. 031 25 g / mL,and MBC was 0. 062 50 g / mL; to Staphylococcus aureus,and MIC was 0. 062 50 g / mL,and MBC was 0. 125 00 g / mL; to Proteus,MIC was 0. 062 50 g / mL,and MBC was 0. 125 00 g / mL. [Conclusions]The extract of Achillea millefolium extracted by different methods have some antibacterial effects.