Effects of dendritic cells transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response

AIM:To investigate the effects of dendritic cells(DCs)transfected with full-length wild-type p53 and stimulatedby gastric cancer lysates on immune response.METHODS:The wild-type p53was transduced to DCs withadenovirus,and the DCs were stimulated by gastric cancerlysates.The surface molecules(B7-1,B7-2,MHC-Ⅰ,MHC-Ⅱ)of all DCs were detected by FACS,and the ability of the DCsto induce efficient and specific immunological response inanti-51Cr-labeled target cells was studied.BALB/c miceinjected with DCs and Mk28 were established,and CTLresponse in mice immunized with Lywt-p53DC was evaluated.Tumor-bearing mice were treated with Lywt-p53DC.RESULTS:The surface molecules of Lywt-p53DC had a highexpression of B7-1(86.70±0.07%),B7-2(18.77±0.08%),MHC-Ⅰ(87.20±0.05%)and MHC-Ⅱ(56.70±0.07%);Tlymphocytes had a specific CTL lysis ability induced by Lywt-p53DC;the CTL lysis rate was as high as 81%.The immuneprotection of Lywtp-53DC was obvious,the tumor diameterin Lywtp-53DC group was 3.10±0.31 mm,2.73±0.23mm,3.70±0.07 mm on d 13,16 and 19,respectively,whichwere smaller than control,DC,wtp53DC and LyDC group(P<0.05).Tumor growth rate in Lywtp53DC group wasslower than that in other groups(P<0.05).CONCLUSION:DCs transfected with wild-type p53 andstimulated by gastric cancer lysates have specific CTL killingactivity.