A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation

被引：0
|
作者：
Jian Wei LI
Fang TIAN
Chen WANG
and Chun Li BAI(Institute of Chemistry
机构：
来源：
Chinese Chemical Letters | 1997年 / 01期
关键词：
DNA; A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation;
D O I：
暂无
中图分类号：
O629 [天然化合物];
学科分类号：
070303 ; 081704 ;
摘要：
λ-DNA Hind III was denatured by HCI, renatured by NaOH, and digested by DNase 1 sequentially. The produced mixture was separated with Sephadex G200 column, and the chromatograph showed a very weak peak of one component resistant to the digestion of DNase 1.The results of AFM studies on this component indicated that an unusual form of DNA was present,with the height 13 times that of double-stranded DNA. By comparison with known results, it is suggested that this is a multi-stranded DNA other than simple supercoils.
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页码：49 / 52
页数：4
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