Isolation, Identification and Pathogenicity Evaluation of Pathogens of Soybean Bacterial Diseases

In order to research soybean bacterial diseases in Heilongjiang Province,a bacterial strain was isolated from diseased leaves of Suinong 14 collected from Harbin Yanjiagang farm. The pathogen had been carried out antibiotic resistance identification,molecular identification,re-inoculation identification and pathogenicity identification respectively. By Gram staining,pathogen inoculation test,16 S r DNA molecular identification,the pathogen was determined as Pseudomonas syringae and named Psgneau 2. The isolated strain presented resistance to 25,50 and 100 μg/m L ampicillin and carbenicillin,but susceptible to spectinomycin,rifampicin,kanamycin,gentamicin,tetracycline and chloramphenicol. Ten soybean varieties were inoculated with Psgneau 2,in which Suinong 14 was more susceptible than other varieties and Hefeng 35 presented higher resistance to Psgneau 2.