Effects of Mogroside V on Glucose and Lipid Metabolism in High Fat Diet Mice

[Objectives] To explore the effects and mechanism of mogroside V(MV) on glucose and lipid metabolism in high-fat diet(HFD) mice. [Methods] The experiment fed mice with high-fat diet for 8 weeks, and 40 mice with successful modeling were randomly divided into normal group, model group, and MV dose group(100, 200 mg/kg), with 10 mice in each group. From the ninth week, the MV dose group was given intragastric administration, and the normal group and the model group were given an equal volume of distilled water by intragastric administration for 6 weeks, then killed and blood samples and livers were collected. Serum triglycerides(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), free fatty acids(FFA), Advanced glycation end products(AGE-P)-peptides(AGE-P) and glycosylated hemoglobin(HbA1 c) content, and TG and hepatic glycogen content in liver were detected by biochemical method. Fasting blood glucose(FBG) was measured by glucose oxidase method. The fasting serum insulin(FINS) content was detected by enzyme-linked immunosorbent assay(ELISA), and the insulin resistance index(HOMA-IR) was calculated. Oil red O staining was used to observe the fat deposition in liver tissue. [Results] MV(100, 200 mg/kg) dose groups could significantly down-regulate the contents of TC, TG, LDL-C, FBG, FINS, AGE-P and HbA1 c and HOMA-IR, and up-regulate HDL-C and hepatic glycogen content and reduce the fat deposits. [Conclusions] The mechanism of MV regulating glucose and lipid metabolism in mice may be related to the regulation of insulin resistance.