MiR-106b-5p调控老年骨质疏松性骨折的分子网络

被引:3
|
作者
王海龙 [1 ]
李龙 [1 ]
伊力哈木托合提 [1 ]
刘旭 [2 ]
那次克道尔吉 [2 ]
陈洪涛 [2 ]
崔泳 [3 ]
机构
[1] 新疆医科大学第七附属医院骨科
[2] 新疆医科大学第六附属医院运动损伤科
[3] 新疆医科大学第五附属医院骨科
关键词
骨质疏松性骨折; miR-106b-5p; PI3K-Akt; 转化生长因子β; 差异表达;
D O I
暂无
中图分类号
R580 []; R683 [骨折、骨的损伤];
学科分类号
摘要
背景:骨质疏松性骨折是与年龄有关的疾病,由于人口老龄化,其发病率和患病率不断上升。目的:鉴定miRNA与老年骨质疏松性骨折有关。方法:通过limma包分析GSE93883中骨质疏松性骨折相关的差异表达miRNA;通过RAID数据库预测差异表达miRNA的靶标基因,并通过PPI(蛋白质-蛋白质相互作用)网络识别关键miRNA及靶标基因;富集分析识别靶标基因参与的生物功能和信号通路。最后,收集5例老年骨质疏松性骨折和5例老年非骨质疏松性骨折患者的骨组织,利用qPCR和Western blot验证关键miRNA及信号通路。结果与结论:(1)共获得21个骨质疏松性骨折相关的差异表达miRNA并预测得到530个靶标基因;通过PPI网络鉴定miR-106b-5p为关键调控基因,其靶标基因主要与MAPK活性的激活等生物功能以及PI3K-Akt和转化生长因子β信号通路相关;(2)qPCR结果验证了miR-106b-5p在老年骨质疏松性骨折骨组织中的表达较对照骨组织显著下调(P <0.05);Western blot结果显示PI3K-Akt和转化生长因子β信号通路在老年骨质疏松性骨折中被激活;(3)提示:miRNA参与老年骨质疏松性骨折,并与PI3K-Akt和转化生长因子β信号通路相关。miR-106b-5p可能抑制骨质疏松性骨折的发生,并成为潜在的标志物和治疗靶标。
引用
收藏
页码:239 / 244
页数:6
相关论文
共 35 条
  • [1] Bench-to-bedside strategies for osteoporotic fracture: from osteoimmunology to mechanosensation[J]. Yong Xie,Licheng Zhang,Qi Xiong,Yanpan Gao,Wei Ge,Peifu Tang.Bone Research. 2019(03)
  • [2] MiR-21 nanocapsules promote early bone repair of osteoporotic fractures by stimulating the osteogenic differentiation of bone marrow mesenchymal stem cells[J] . Xiaolei Sun,Xueping Li,Hongzhao Qi,Xin Hou,Jin Zhao,Xubo Yuan,Xinlong Ma.Journal of Orthopaedic Translation . 2020 (prep)
  • [3] The Clinical Potential of Circulating miRNAs as Biomarkers: Present and Future Applications for Diagnosis and Prognosis of Age-Associated Bone Diseases[J] . Michela Bottani,Giuseppe Banfi,Giovanni Lombardi.Biomolecules . 2020 (4)
  • [4] Serum microRNAs as novel biomarkers for osteoporotic vertebral fractures[J] . Patryk Zarecki,Matthias Hackl,Johannes Grillari,Miguel Debono,Richard Eastell.Bone . 2020 (C)
  • [5] Deciphering the Underlying Mechanism of Eucommiae Cortex against Osteoporotic Fracture by Network Pharmacology[J] . Yongming Shuai,Zhili Jiang,Qiuwen Yuan,Shuqiang Tu,Fanhui Zeng,Antonella Fioravanti.Evidence-Based Complementary and Alternative Medicine . 2020
  • [6] A validated miRNA signature for the diagnosis of osteoporosis related fractures using SVM algorithm classification
    Tang, Xiaolin
    Bai, Yinshan
    Zhang, Zhiming
    Lu, Jianlin
    [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 20 (03) : 2209 - 2217
  • [7] VEGFA From Early Osteoblast Lineage Cells (Osterix+) Is Required in Mice for Fracture Healing[J] . Buettmann Evan G,McKenzie Jennifer A,Migotsky Nicole,Sykes David Aw,Hu Pei,Yoneda Susumu,Silva Matthew J.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research . 2019 (9)
  • [8] Osteoporotic Fracture Guidelines and Medical Education Related to the Clinical Practices: A Nationwide Survey in China
    Lu, Jin
    Ren, Zhong
    Liu, Xun
    Xu, You-jia
    Liu, Qiang
    [J]. ORTHOPAEDIC SURGERY, 2019, 11 (04) : 569 - 577
  • [9] Current Progress on MicroRNA-Based Gene Delivery in the Treatment of Osteoporosis and Osteoporotic Fracture
    Sun, Xi
    Guo, Qi
    Wei, Wenhua
    Robertson, Stephen
    Yuan, Ying
    Luo, Xianghang
    [J]. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, 2019, 2019
  • [10] The Therapeutic Potential of MicroRNAs as Orthobiologics for Skeletal Fractures
    Hadjiargyrou, Michael
    Komatsu, David E.
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2019, 34 (05) : 797 - 809