Monoclonal Antibodies Recognizing HIV-1 gp41 Could Inhibit Env-Mediated Syncytium Formation

被引：1
|
作者：
张耿
陈应华
机构：
[1] Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China
[2] Laboratory of Immunology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China ,Laboratory of Immunology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
来源：
Tsinghua Science and Technology | 2005年 / 04期
基金：
中国国家自然科学基金;
关键词：
HIV-1; monoclonal antibody; cell fusion;
D O I：
暂无
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Some monoclonal antibodies (mAbs) could inhibit infection by HIV-1. In this study, four mAbs against HIV-1 gp41 were prepared in mice. All four mAbs could bind to the recombinant soluble gp41 and recognize the native envelope glycoprotein gp160 expressed on the HIV-Env+ CHO-WT cell in flow cytometry analysis. Interestingly, the results show that all four mAbs purified by affinity chromatography could inhibit HIV-1 Env-mediated membrane fusion (syncytium formation) by 40%-60% at 10 μg/mL, which implies potential inhibitory activities against HIV-1.
引用
收藏
页码：512 / 516
页数：5
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