Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E.coli by macromolecular cross-linking agent

被引：4

作者：

Liya Zhou ^{[1
,2
]}

Haixia Mou ^{[1
]}

Jing Gao ^{[1
,2
]}

Li Ma ^{[1
]}

Ying He ^{[1
]}

Yanjun Jiang ^{[1
,2
]}

机构：

[1] School of Chemical Engineering and Technology,Hebei University of Technology

[2] Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving,Hebei University of Technology

来源：

Chinese Journal of Chemical Engineering | 2017年 / 25卷 / 04期

关键词：

Immobilization; Enzyme; Biocatalysis; Nitrile hydratase;

D O I：

暂无

中图分类号：

Q814 [酶工程];

学科分类号：

082203 ;

摘要：

Cross-linked enzyme aggregates(CLEAs) of nitrile hydratase(NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time. In this process, egg white was added as protein feeder for facilitating the formation of CLEAs. The optimal conditions of the immobilization process were determined. Michaelis constants(Km) of free NHase and NHase CLEAs were also determined. The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart. When exposed to high concentrations of acrylamide, NHase CLEAs also exhibited effective catalytic activity.

引用

页码：487 / 492

页数：6

共 25 条

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