Establishment of the Determination Methods of Total Anthraquinone Content in Different Processed Products of Rhubarb

[Objectives] Ultraviolet spectrophotometry was established for the determination of total content of anthraquinone in different processed products of rhubarb. [Methods] Emodin was used as the control substance, and its color was developed by magnesium acetate methanol solution with the concentration of 1%, the absorbance was determined at the wavelength of 516 nm, the content of total anthraquinone in different processed products of rhubarb was determined to compare the content differences. [Results] The regression equation was y=0.045 2x+0.001 5, R2=0.999 1. Emodin had a good linear range in the concentration of 0.98-23.52 μg/mL, with high accuracy, good repeatability and stable detection results. This method revealed the content changes of total anthraquinone in rhubarb before and after processing. [Conclusions] This method could be used as the quality control method of different processed products of rhubarb, and provided reference for the comprehensive development and utilization of rhubarb resources in the future.