Extraction Process and Content Determination of Total Flavonoids in Lonicera japonica

[Objectives] To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination. [Methods] The total flavonoids of L. japonica were extracted by reflux extraction method. Through a single factor experiment, the effects of extraction method, extraction solvent concentration, extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of L. japonica. [Results] The optimal extraction process of the total flavonoids of L. japonica was 70% ethanol, 1∶30 of solid-to-liquid ratio, and 1.0 h of reflux extraction time. [Conclusions] This method can effectively determine the content of total flavonoids in L. japonica and is expected to provide a certain scientific basis for the study of the quality standard of L. japonica. This method has high reproducibility. It is stable and feasible in extraction of total flavonoids from L. japonica.