Study on Backflow Extraction Technology of Total Saponins from Stem Base of Panax notoginseng(Burk) F.H.

[Objective]To optimize the methods for content determination and backflow extraction technology of total saponins from stem base of Panax notoginseng(Burk) F.H.[Method]The content of total saponins was determined by the methods of vanillin-perchloric acid and the extraction technology was optimized by the content of total saponins.[Result]Panax saponin Rg 1 presented good linear relationship over the range of 0.008 3 to 0.049 6 mg /ml(R =0.999 4).The average recovery was 98.6% with the RSD of 3.54%(n =4).The optimal solvent backflow extraction conditions were as follows:P.notoginseng stem base was soaked for 12 h in 60% ethanol at the solid-liquid ratio of 1:10(g /ml) and extracted for 3 times for 2 h each time.[Conclusion]This method is simple and feasible,highly accurate and precise.The extraction process is economical,reasonable and deserved popularizing all the more.