Comparative Several Extraction Methods of Genomic DNA from Tripterygium wilfordii Hook. f. Leaves

被引：0
|
作者：
XING Jian-hong1
2. Fujian University of Traditional Chinese Medicine
机构：
来源：
Medicinal Plant | 2010年 / 03期
关键词：
Tripterygium wilfordii Hook. f; DNA extraction; Different extraction methods; China;
D O I：
暂无
中图分类号：
S567.19 [];
学科分类号：
1008 ;
摘要：
[Objective] Comparison of the extraction methods of genomic DNA from the leaves of Tripterygium wilfordii Hook. f. [Method] With leaves of Tripterygium wilfordii Hook. f. as tested material, the genomic DNA was extracted using five different methods: CTAB method, modified CTAB method, SDS method, high salt and low pH method and ROSE method. The purity of the DNA was examined by agarose gel electrophoresis, OD260/OD280 and RAPD amplification, and the yield of the DNA was estimated by OD260. [Result] Modified CTAB method was the optimum one for DNA extraction. The ratio of OD260 and OD280 of the genomic DNA extracted by modified CTAB method was 1.869-1.903, and showed good performance in RAPD analysis. [Conclusion] The modified CTAB method is an optimum method for the extraction of genomic DNA from Tripterygium wilfordii Hook. f.
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页码：12 / 14 +18
页数：4
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