Development of a sensitive and reliable droplet digital PCR assay for the detection of ‘Candidatus Liberibacter asiaticus'

被引：0

作者：

ZHONG Xi ^{[1
]}

LIU Xue-lu ^{[1
]}

LOU Bing-hai ^{[2
]}

ZHOU Chang-yong ^{[1
]}

WANG Xue-feng ^{[1
]}

机构：

[1] National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University

[2] Guangxi Key Laboratory of Citrus Biology, Guangxi Academy of Specialty Crops

来源：

Journal of Integrative Agriculture | 2018年 / 17卷 / 02期

关键词：

citrus Huanglongbing; early diagnosis; droplet digital PCR;

D O I：

暂无

中图分类号：

S436.66 [柑桔类病虫害];

学科分类号：

090401 ; 090402 ;

摘要：

Citrus Huanglongbing(HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR(ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus’(Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that dd PCR was superior to quantitative PCR(qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples(46.15%) with high Ct value(>35) were positive by dd PCR. This methodology showed great potential for early HLB infection diagnosis.

引用

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页码：483 / 487

页数：5

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