Determination of Polysaccharides in Ginseng Stem Leaves by Phenol-Sulfuric Acid Method

[Objective] To optimize the factor conditions of the polysaccharides in ginseng stem leaves by phenol-sulfuric acid method. [Method] Polysaccharides contents in ginseng stem leaves were detected by UV Spectrophotometry; detection condition was optimized by orthogonal test. [Result] The optimal wavelength was 485 nm for the determination of polysaccharides contents in ginseng stem leaves by phenol-sulfuric acid method. The optimal color condition was A1B2C1, which was 1.0 ml of 6% phenol, 6.0 ml concentrated sulfuric acid, and 25 min reaction time. Crude polysaccharides had good linear relationship with absorbency between 10 and 70 μg/ml. The regression equation was Y=0.013 2X-0.085 7; correlation coefficient R was 0.999 6; recovery rate of test method was 100.36%, and RSD was 1.26%. The coloured solution had good stability within 120 min. [Conclusion] This method was accurate and reliable, and provided references for the further development and utilization of ginseng stem leaves.