Optimization of Ultrasonic-assisted Extraction of Cultured Cordyceps militaris Polysaccharide by Response Surface Methodology

Objective To optimize technique for extracting Cordyceps militaris Polysaccharide Method By adopting Response Surface Method and Center-rotated test, the duration of ultrasonic extraction, temperature and the ratio of liquid to solids effect on extraction rate was researched.Result The optimal extraction conditions were figured out: 80 ℃, the ratio of liquid to solid was 32 mL/g and 65 min of two-time extraction. Theoretically, the extraction rate can be 3.89%. Conclusion The Response Surface Method can optimize the techniques for ultrasonically extracting Cordyceps militaris polysaccharide, provide the optimum process parameters and lay the foundation for further research.