Influence of Stent Implantation on the Expression of PCNA and Apoptosis in Injured Vascular Smooth Muscle Cells

被引：0
|
作者：
刘英梅
张旭明
伍卫
王景峰
陈筱潮
机构：
[1] P R China The grant from Sanitation Department Science Foundation of Guangdong
[2] Department
[3] Sun yat - Sen Memorial Hospital. Sun Yat - Sen University
[4] of cardiology
[5] Guangzhou 510120
来源：
South China Journal of Cardiology | 2002年 / 02期
关键词：
Restenosis Stent Vascular smooth muscle cells (VSMC) Proliferating cell nu-clear antigen (PCNA) Apoptosis;
D O I：
10.16268/j.cnki.44-1512/r.2002.02.009
中图分类号：
R543 [血管疾病];
学科分类号：
1002 ; 100201 ;
摘要：
Objectives To evaluate the impact of stent implantation on proliferation and apop-tosis in injured media vascular smooth muscle cells (VSMC) and to explore the mechanism of restenosis after stent implantation. Methods Fifty male New Zealand rabbits were randomized into two groups, including balloon group and stent group. Control group was set up. The samples were harvested on 3, 7, 14, 28, 56 days after operation and the following investigation was carried out: (1) Assessing the expression of proliferating cell nuclear antigen (PCNA) of media VSMC by the method of immunohistochemistry; (2) Analyzing apoptosis of media VSMC by DNA agarose gel electrophoresis and TUNEL technique. Results The expression of PCNA and apoptosis in stent and balloon groups were markedly increased compared with control groups. (1) Stent group induced significant increased expression of PCNA in the media VSMC compared with balloon group on 3 to 28 days. On day 7, the positive rates of PCNA were 24. 36±0. 55 % vs 18. 74±1. 09 %
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收藏
页码：99 / 103
页数：5
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