Periodontal Ligament Cells Cultured on the Tricalcium Phosphate/Chitosan Scaffolds in vivo and in vitro

被引：0
|
作者：
钟志华 [1 ,2 ]
施斌 [1 ]
机构：
[1] School of Stomatology, Wuhan University
[2] Department of Stomatology, Jingzhou Central Hospital
来源：
Journal of Wuhan University of Technology(Materials Science) | 2014年 / 01期
基金：
中国国家自然科学基金;
关键词：
chitosan; TCP; scaffold; HPLCs; tissue engineering;
D O I：
暂无
中图分类号：
R781.4 [牙周病];
学科分类号：
100302 ;
摘要：
To search suitable scaffolds for periodontal tissue regeneration, different ratios of tricalcium phosphate/chitosan(TCP/chitosan) scaffolds were prepared through a freeze-drying process. Human periodontal ligament cells(HPLCs) were incubated on the scaffolds in vitro. Cells were cultured on the scaffolds, detected by scanning electron microscopy(SEM).HPLCs were analyzed by MTT assay and alkaline phosphatase(ALP) activity detection. HPLCs were seeded onto the scaffolds. Then these scaffolds with HPLCs were implanted subcutaneously into athymic mice. The state of periodontal tissue regeneration was detected after 4 weeks. As the ratio of TCP increased, TCP/chitosan scaffolds accelerated HPLCs proliferation significantly higher than the pure chitosan scaffold. HPLCs produced the cementoid tissue in vivo. The periodontal tissue regeneration engineering is able to be applied with TCP/chitosan scaffolds.
引用
收藏
页码：174 / 179
页数：6
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