Microbial Diversity in Rhizosphere Soil of Cotinus coggygria Based on High Throughput Sequencing

In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park, the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively, were conducted by high throughput sequencing(Illumina Mi Seq). The results showed that the fungal diversity in the rhizosphere soil samples of C. coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016, and it was higher in the rhizosphere soil of healthy C. coggygria in Xunlupo than that in diseased ones in 2018. Verticillium dahliae, which is the causal agent of C. coggygria wilt, was detected in five soil samples. In 2018, the bacterial diversity in the rhizosphere soil of diseased C. coggygria in Xunlupo was the lowest, while it was the highest in the rhizosphere soil of healthy C. coggygria under Platycladus orientalis in Langfengting.