This paper is devoted to the study of existence,uniqueness and non-degeneracy of positive solutions of semi-linear elliptic equations.A necessary and sufficient condition for the existence of positive solutions to problems is given.We prove that if the uniqueness and non-degeneracy results are valid for positive solutions of a class of semi-linear elliptic equations,then they are still valid when one perturbs the differential operator a little bit.As consequences,some uniqueness results of positive solutions under the domain perturbation are also obtained.