Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR

被引：0
|
作者：
Consortium for Influenza Study at Shanghai(YAN An1
2 Shanghai Institutes of Biological Sciences
Center of Bioinformatics
Lab of Synthetic Biology
Institute Pasteur)
3 National Engineering Research Center for Biochip Technology
4 Shanghai Center for Bioinformation Technology
5 School of Life Sciences
6 School of Life Sciences and Technology
7 Cancer Institute
8 National Engineering Research Center for Antibody Drugs
9 Zhujiang Hospical
10 Shanghai Municipal Center for Disease Control & Prevention
11 School of Life Sciences and Technology
12 National Institute for Parasitic Diseases
13 Shanghai Institute of Organic Chemistry
14 Shanghai Veterinary Research Institute
机构：
来源：
Science Bulletin | 2009年 / 13期
关键词：
influenza A virus; HA gene; H1 and H3 subtypes; specific amplification by RT-PCT; DNA sequencing;
D O I：
暂无
中图分类号：
R181.3 [流行病学各论]; R450 [];
学科分类号：
100215 ; 100401 ;
摘要：
The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential.
引用
收藏
页码：2164 / 2167
页数：4
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