Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR

被引：0
|
作者：
Consortium for Influenza Study at Shanghai(YAN An1
2 Shanghai Institutes of Biological Sciences
Center of Bioinformatics
Lab of Synthetic Biology
Institute Pasteur)
3 National Engineering Research Center for Biochip Technology
4 Shanghai Center for Bioinformation Technology
5 School of Life Sciences
6 School of Life Sciences and Technology
7 Cancer Institute
8 National Engineering Research Center for Antibody Drugs
9 Zhujiang Hospical
10 Shanghai Municipal Center for Disease Control & Prevention
11 School of Life Sciences and Technology
12 National Institute for Parasitic Diseases
13 Shanghai Institute of Organic Chemistry
14 Shanghai Veterinary Research Institute
机构：
来源：
Science Bulletin | 2009年 / 13期
关键词：
influenza A virus; HA gene; H1 and H3 subtypes; specific amplification by RT-PCT; DNA sequencing;
D O I：
暂无
中图分类号：
R181.3 [流行病学各论]; R450 [];
学科分类号：
100215 ; 100401 ;
摘要：
The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential.
引用
收藏
页码：2164 / 2167
页数：4
相关论文
共 50 条
  • [1] Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
    Yan An
    Ding GuoHui
    Zhou ZhenFeng
    Dong Hui
    Zhang YaKun
    Zhu Lei
    He YunGang
    Zhang GuoQing
    Li YiXue
    Sun Bing
    Huang Zhong
    Lan Ke
    Jin Li
    Wang HongYan
    Wang XiaoNing
    Yang Zhong
    Zhong Yang
    Dai JianXin
    Guo YaJun
    Wang Hao
    Che XiaoYan
    Wu Fan
    Yuan ZhenGan
    Zhang Xi
    Cao ZhiWei
    Zhou XiaoNong
    Zhou JiaHai
    Ma ZhiYong
    Tong GuangZhi
    Zhao GuoPing
    Jin WeiRong
    Xiong Hui
    CHINESE SCIENCE BULLETIN, 2009, 54 (13): : 2164 - 2167
  • [2] Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
    Consortium for Influenza Study at ShanghaiYAN AnDING GuoHuiZHOU ZhenFengDONG HuiZHANG YaKunZHU LeiHE YunGangZHANG GuoQingLI YiXueSUN BingHUANG ZhongLAN KeJIN LiWANG HongYanWANG XiaoNingYANG ZhongZHONG YangDAI JianXinGUO YaJunWANG HaoCHE XiaoYanWU FanYUAN ZhenGanZHANG XiCAO ZhiWeiZHOU XiaoNongZHOU JiaHaiMA ZhiYongTONG GuangZhiZHAO GuoPingJIN WeiRong XIONG Hui ShanghaiMOST Key Laboratory for Disease and Health GenomicsChinese National Human Genome Center at ShanghaiShanghai China Shanghai Institutes of Biological SciencesChinese Academy of SciencesLab of Computational GenomicsCASMPG Partner Institute of Computational Biology
    Center of BioinformaticsKey Lab of System Biology
    Lab of Synthetic BiologyInstitute of Plant Physiology Ecology
    Institute PasteurShanghai China National Engineering Research Center for Biochip TechnologyShanghai Biochip CoLtdShanghai China Shanghai Center for Bioinformation TechnologyShanghai China School of Life SciencesFudan UniversityShanghai China School of Life Sciences and TechnologySouth China University of TechnologyGuangzhou China Cancer InstituteSecond Military Medical UniversityShanghai China National Engineering Research Center for Antibody DrugsShanghai China Zhujiang HospicalSouthern Medical UniversityGuangzhou China Shanghai Municipal Center for Disease Control PreventionShanghai China School of Life Sciences and TechnologyTongji UniversityShanghai China National Institute for Parasitic DiseasesChinese Center for Disease Control and PreventionShanghai China Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai China Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai China
    Chinese Science Bulletin, 2009, 54 (13) : 2164 - 2167
  • [3] Detection of equina influenza virus by haemagglutinin (HA) gene based RT-PCR
    Gupta, AK
    Sharma, V
    Yadav, MP
    INDIAN VETERINARY JOURNAL, 2001, 78 (05): : 382 - 385
  • [4] Typing and subtyping of influenza viruses and respiratory syncytial viruses by multiplex RT-PCR
    Yamada, A
    Lam, LY
    Tam, JS
    OPTIONS FOR THE CONTROL OF INFLUENZA V, 2004, 1263 : 381 - 385
  • [5] Development of multiplex RT-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens
    Chang, Hee Kyoung
    Park, Jeung Hyun
    Song, Min-Suk
    Oh, Taek-Kyu
    Kim, Seok-Young
    Kim, Chul-Jung
    Kim, Hyunggee
    Sung, Moon-Hee
    Han, Heon-Seok
    Hahn, Youn-Soo
    Choi, Young-Ki
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (06) : 1164 - 1169
  • [6] Neuraminidase subtyping of human influenza A viruses by RT-PCR and its application to clinical isolates
    Takao, S
    Shimazu, Y
    Fukuda, S
    Kuwayama, M
    Miyazaki, K
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2002, 55 (06) : 204 - 205
  • [7] Validation of a RT-PCR Gene Expression Assay for Subtyping Lung Tumor Samples
    Faruki, H.
    Miglarese, M. R.
    Mayhew, G.
    Eisenberg, M.
    Horten, B.
    Anderson, S.
    Kam-Morgan, L.
    Yan, J.
    Parker, S.
    Hayes, D. N.
    Perou, C. M.
    Lai-Goldman, M.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2014, 16 (06): : 755 - 756
  • [8] Comparison of a nucleoprotein gene based RT-PCR with real time RT-PCR for diagnosis of avian influenza in clinical specimens
    Nagarajan, S.
    Murugkar, H. V.
    Tosh, C.
    Behera, P.
    Khandia, R.
    Jain, R.
    Katare, M.
    Syed, Z.
    Tripati, S.
    Dubey, S. C.
    RESEARCH IN VETERINARY SCIENCE, 2012, 93 (01) : 504 - 507
  • [9] Multiplex RT-PCR and Indirect Immunofluorescence Assays for Detection and Subtyping of Human Influenza Virus in Tunisia
    Ben M'hadheb, Manel
    Harrabi, Myriam
    Souii, Amira
    Jrad-Battikh, Nadia
    Gharbi, Jawhar
    CURRENT MICROBIOLOGY, 2015, 70 (03) : 324 - 329
  • [10] Multiplex RT-PCR and Indirect Immunofluorescence Assays for Detection and Subtyping of Human Influenza Virus in Tunisia
    Manel Ben M’hadheb
    Myriam Harrabi
    Amira Souii
    Nadia Jrad-Battikh
    Jawhar Gharbi
    Current Microbiology, 2015, 70 : 324 - 329
12345