Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro

被引：0

作者：

ZHANG Yu1

2.Department of Radiotherapy

机构：

来源：

Chemical Research in Chinese Universities | 2009年 / 25卷 / 01期

基金：

中国国家自然科学基金;

关键词：

hTERT; HSV-TK; Adenovirus; Nasopharyngeal carcinoma;

D O I：

暂无

中图分类号：

R739.63 [咽肿瘤];

学科分类号：

100214 ;

摘要：

Human telomerase reverse transcriptase(hTERT) activity was detected in human nasopharyngeal carci-noma cell(CNE) but not in human normal lung fibroblas t(CCD-11Lu).Recombinant adenoviruses Ad-CMV-TK-enh and Ad-hTERT-TK-enh were constructed and infected into normal fibroblasts and nasopharyngeal carcinoma cells.Ad-CMV-TK-enh with 100 μmol/L of ganciclovir(GCV) caused 87% of CCD-11Lu cells death and 91% of CNE cells death,Ad-hTERT-TK-enh with 100 μmol/L of GCV caused 24% of CCD-11Lu cells death and 79% of CNE cells death.These results indicate that the Ad-hTERT-TK-enh with GCV may be a useful method in suppressing tumor growth in targeted nasopharyngeal carcinoma gene therapy.

引用

页码：60 / 63

页数：4

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