IMMOBILIZED ASPERGILLUS ORYZAE CELLS AND THEIR ACTIVITIES

被引：0
|
作者：
宋正孝 [1 ]
李晓敏 [1 ]
王诤 [1 ]
彭英 [1 ]
机构：
[1] 天津大学化学工程系
来源：
Transactions of Tianjin University | 1997年 / 02期
关键词：
固定化; 米曲霉; 氨基酰化酶; 光学拆分;
D O I：
暂无
中图分类号：
Q556 [水解酶];
学科分类号：
摘要：
采用戊二醛－明胶－乙二胺将氨基酰化酶交联固定在米曲霉细胞上，应用正交实验设计优选出相应的固定化条件，制备出具有较高反应活性和稳定性，适用于拆分ＤＬ－氨基酸的固定化细胞，并就ｐＨ、温度、离子浓度、底物浓度等因素对固定化细胞反应活性的影响进行了实验研究．分别在固定化细胞柱（ＩＣＣ）和新型固定化细胞－膜耦联反应器（ＣＩ－ＣＢＭＲ）中进行了连续光学拆分Ｎ－乙酰－ＤＬ－丙氨酸的实验研究，结果表明该固定化细胞具有良好的工业应用前景．
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页数：5
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