Suppression of bcl-2 Gene by RNA Interference Increases Chemosensitivity to Cisplatin in Nasopharyngeal Carcinoma Cell Line CNE1

被引：0
|
作者：
Zhi-Hua YIN
机构：
来源：
Acta Biochimica et Biophysica Sinica | 2004年 / 11期
关键词：
RNA interference; nasopharyngeal carcinoma; bcl-2; cisplatin;
D O I：
暂无
中图分类号：
R739.63 [咽肿瘤];
学科分类号：
摘要：
To explore the effect of suppressing BCL-2 expression using RNA interference (RNAi) technique in nasopharyngeal carcinoma cell line CNE1. CNE1 cell lines stably expressing shRNAs targeted bcl-2 and GL3 gene were established and gene expression inhibition was assessed by Western blotting analysis. The effect of suppressing bcl-2 by RNAi on cell growth was studied, the apoptosis induction and the sensitization of CNE1 cells to cisplatin were quantified by MTT assay and flow cytometry. The results showed that: stable transfection of CNE1
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页码：749 / 753
页数：5
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