Synthesis and In-Vitro Antimycobacterial Evaluation of N-Arylidene-5-Aryl-1,3,4-Oxadiazol-2-Amines Derivatives

A series of N-arylidene-5-aryl-1,3,4-oxadiazol-2-amines (2a-d, 3a-d & 4a-d) were synthesized with the help of aryl acids, semicarbazide, and aryl benzaldehyde. These synthesized compounds were characterized by elemental analysis, FT-IR, 1H NMR, and mass spectral data analysis. The title compounds were evaluated as in-vitro antimycobacterial agents against Mycobacterium tuberculosis H37RV via the Microplate Alamar Blue Dye Assay (MABA) method. The in-vitro antimycobacterial result revealed that the most active compounds (2c, 2d, 3c, 3d, 4d & 4d) showed a minimum inhibitory concentration (MIC) value of 6.25 μg/ml when compared with the standard drugs Ciprofloxacin, Pyrazinamide (3.125 μg/ml), and Streptomycin (6.25 μg/ml). Most of the title compounds exhibited MIC values ranging from 6.25 to 12.5 μg/ml. Compound 3a was found to be the least effective compound. Also, the electron-withdrawing group (Cl & NO2) on the phenyl ring was found to be more effective than the electron-releasing groups (OH). All the synthesized compounds encouraged us to continue to progress and improve other novel compounds with effective antimycobacterial potential.