Ultrafast transient absorption spectra and kinetics of human blue cone visual pigment at room temperature

被引：0
|
作者：
Krishnamoorthi, Arjun [1 ]
Salom, David [2 ,3 ]
Wu, Arum [2 ,3 ]
Palczewski, Krzysztof [2 ,3 ,4 ,5 ,6 ]
Rentzepis, Peter M. [1 ]
机构：
[1] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA
[2] Univ Calif Irvine, Gavin Herbert Eye Inst, Dept Ophthalmol, Irvine, CA 92697 USA
[3] Univ Calif Irvine, Sch Med, Dept Ophthalmol, Irvine, CA 92697 USA
[4] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA
[5] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA
[6] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2024年 / 121卷 / 41期
关键词：
blue cone opsin; cone photo-intermediates; cone visual pigments; phototransduction; ultrafast spectroscopy; PROTEIN-COUPLED RECEPTOR; NANOSECOND LASER PHOTOLYSIS; AMINO-ACIDS; MOLECULAR-PROPERTIES; FLASH-PHOTOLYSIS; CHICKEN GREEN; COLOR-VISION; RETINAL ROD; RHODOPSIN; RED;
D O I：
10.1073/pnas.2414037121
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The ultrafast photochemical reaction mechanism, transient spectra, and transition kinetics of the human blue cone visual pigment have been recorded at room temperature. Ultrafast time-resolved absorption spectroscopy revealed the progressive formation and decay of several metastable photo-intermediates, corresponding to the Batho to Meta-II photo-intermediates previously observed with bovine rhodopsin and human green cone opsin, on the picosecond to millisecond timescales following pulsed excitation. The experimental data reveal several interesting similarities and differences between the photobleaching sequences of bovine rhodopsin, human green cone opsin, and human blue cone opsin. While Meta-II formation kinetics are comparable between bovine rhodopsin and blue cone opsin, the transition kinetics of earlier photo-intermediates and qualitative characteristics of the Meta-I to Meta-II transition are more similar for blue cone opsin and green cone opsin. Additionally, the blue cone photo-intermediate spectra exhibit a high degree of overlap with uniquely small spectral shifts. The observed variation in Meta-II formation kinetics between rod and cone visual pigments is explained based on key structural differences.
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页数：10
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