Highly sensitive and aptamer-based electrochemical fipronil biosensor based on primer exchange reaction and catalytic strand displacement dual recycling amplifications

被引:0
|
作者
Liu, Juan [1 ]
Chen, Qianhong [1 ]
Gan, Xiaoyan [1 ]
Yuan, Ruo [1 ]
Xiang, Yun [1 ]
机构
[1] Southwest Univ, Sch Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
Fipronil; Aptamer; Electrochemical biosensor; Primer exchange reaction; Strand displacement reaction; DNA; METABOLITES; MACHINE; SULFONE;
D O I
10.1016/j.aca.2025.343709
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Background: The indiscriminate use of pesticides in agriculture has caused substantial harm to both the environment and human health. Therefore, it is crucial to develop methods for evaluating trace amounts of pesticide residues in food products. This study presents a highly sensitive electrochemical aptasensor to detect fipronil utilizing a new primer exchange reaction (PER)/catalytic strand displacement reaction (CSDR)-integrated dual signal amplification strategy. Results: Upon binding of fipronil to its specific aptamer in the template hairpin/aptamer complex probe, the template hairpin is released and hybridizes with primer to initiate PER for generating a substantial amount of assistant ssDNAs, which trigger subsequent CSDR for cyclically confining numerous methylene blue (MB)-conjugated signal probes onto sensing surface with existence of Klenow (exo-) fragment polymerase (KF) and deoxynucleotide triphosphates (dNTPs). Such many MB labels thus yield considerably enhanced electrochemical current signals, resulting in detection limit of 0.054 nM for fipronil within the dynamic range of 0.1 nM-1 mu M. Moreover, the sensing method is highly selective and also effective for detecting low concentrations of fipronil in cabbage samples. Significance: With the successful demonstration of the significant signal amplification capability of our PER/CSDR methodology for fipronil, such electrochemical biosensor can be further developed as a robust sensing system for sensitive detection of various small molecules and other biomarkers by substituting the corresponding aptamers for different applications.
引用
收藏
页数:7
相关论文
共 50 条
  • [31] Highly sensitive biosensor based on aptamer and hybridization chain reaction for detection of cadmium ions
    Xu, Mingming
    Peng, Yu
    Yang, Hualin
    Zhou, Yu
    LUMINESCENCE, 2022, 37 (04) : 665 - 671
  • [32] Coupling strand extension/excision amplification with target recycling enables highly sensitive and aptamer-based label-free sensing of ATP in human serum
    Xu, Lin
    Jiang, Bingying
    Zhou, Wenjiao
    Yuan, Ruo
    Xiang, Yun
    ANALYST, 2020, 145 (02) : 434 - 439
  • [33] A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins
    Gao, Huilin
    Bai, Yongchang
    He, Baixun
    Tan, Cherie S.
    BIOSENSORS-BASEL, 2022, 12 (12):
  • [34] A highly sensitive of electrochemical biosensor based on new aptamer sequence for 17α-ethinylestradiol detection in water
    Rozi, Normazida
    Abu Hanifah, Sharina
    Hassan, Riyadh Abdulmalek
    Karim, Nurul Huda Abdul
    Ikeda, Masato
    Mansor, Nurul Nasuha
    Mun, Tang Kok
    Arputheraj, Teffanie A. P.
    MICROCHEMICAL JOURNAL, 2024, 205
  • [35] Ultrasensitive Aptamer-Based Multiplexed Electrochemical Detection by Coupling Distinguishable Signal Tags with Catalytic Recycling of DNase I
    Tang, Dianping
    Tang, Juan
    Li, Qunfang
    Su, Biling
    Chen, Guonan
    ANALYTICAL CHEMISTRY, 2011, 83 (19) : 7255 - 7259
  • [36] Aptamer-based exonuclease protection and enzymatic recycling cleavage amplification homogeneous assay for the highly sensitive detection of thrombin
    Xue, Qingwang
    Zhang, Ge
    Wang, Lei
    Jiang, Wei
    ANALYST, 2014, 139 (12) : 3167 - 3173
  • [37] Sensitive and Electrochemical Detection of Telomerase Activity Based on the Signal Amplification of Strand Displacement Reaction
    Ma, Yanrong
    Jiang, Shengnan
    Jin, Yan
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2021, 42 (03): : 745 - 751
  • [38] A homogeneous fluorescent biosensing strategy for highly sensitive detection of DNA based on a programmed entropy-driven strand displacement reaction and DNAzyme for dual recycling amplification
    Li, Yujian
    Ding, Xiaojuan
    Li, Dandan
    Wu, Haiping
    Huang, Wei
    Ding, Shijia
    ANALYTICAL METHODS, 2019, 11 (12) : 1613 - 1619
  • [39] An electrochemical biosensor for highly sensitive detection of microRNA-377 based on strand displacement amplification coupled with three-way junction
    Hu, Rong
    Wang, Ganglin
    Yuan, Rui
    Xu, Yongjie
    Yu, Tianxiao
    Zhong, Liang
    Zhou, Qin
    Ding, Shijia
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2017, 789 : 160 - 166
  • [40] Dual signal amplification for highly sensitive electrochemical detection of uropathogens via enzyme-based catalytic target recycling
    Su, Jiao
    Zhang, Haijie
    Jiang, Bingying
    Zheng, Huzhi
    Chai, Yaqin
    Yuan, Ruo
    Xiang, Yun
    BIOSENSORS & BIOELECTRONICS, 2011, 29 (01): : 184 - 188