Objectives: This study aimed to investigate the relationship between smartphone use and musculoskeletal pain among healthcare workers of different occupations. The research sought to examine the connection between smartphone habits and the prevalence and severity of musculoskeletal pain, with a focus on work-related and personal smartphone use, physical activity, temporomandibular dysfunction and neck disability. Methods: The study utilized a cross-sectional survey design conducted via Google Forms targeting healthcare workers affiliated with Sisli Hamidiye Etfal Training and Research Hospital. The survey comprised 99 questions assessing demographic and professional information, health conditions, smartphone addiction, physical activity level, musculoskeletal pain, and pain- related disability. Results: A total of 207 hospital staff members' responses were included in the analysis. Participants with higher smartphone addiction scores exhibited a significantly younger mean age and increased smartphone usage post-pandemic. No significant differences were found in physical activity levels between groups. However, those with higher addiction scores reported more frequent pain in the jaw and elbows, greater neck disability score, and a higher prevalence of temporomandibular dysfunction. The study also revealed a significant association between smartphone addiction and multi-site musculoskeletal pain. Conclusion: The findings indicate that smartphone addiction among healthcare workers is associated with specific patterns of musculoskeletal pain, particularly in the jaw and elbows, and increased neck disability scores. The study highlights the need for targeted interventions to promote healthier smartphone habits and mitigate musculoskeletal pain among healthcare professionals.