PLL-g-HPA Hydrogel Loaded Human Umbilical Cord Mesenchymal Stem Cells Promote Burn Wound Healing in Rat Model by Regulating Inflammation Response

被引:0
|
作者
Tian, Linqiang [1 ,2 ]
Wang, Zhaodong [1 ,2 ]
Lei, Tingting [1 ,3 ]
Feng, Lili [1 ]
Li, Yanyan [1 ]
Wang, Kunxi [1 ]
Zhang, Yue [1 ]
Zhang, Chengshu [4 ]
Liu, Jie [1 ]
Xing, Hongxia [3 ]
Ren, Wenjing [5 ]
机构
[1] Xinxiang Med Univ, Affiliated Hosp 3, Henan Key Med Lab Traumat & Orthopaed Res, Xinxiang, Peoples R China
[2] Xinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang, Peoples R China
[3] Xinxiang Med Univ, Affiliated Hosp 3, Dept Neurol, Xinxiang, Peoples R China
[4] Dept Burn & Plast Surg, Gen Hosp, PINGMEI SHENMA Grp, Pingdingshan, Peoples R China
[5] Xinxiang Med Univ, Clin Med Ctr Tissue Engn & Regenerat, Xinxiang, Peoples R China
关键词
rat burn model; wound healing; human umbilical cord mesenchymal stem cells; p-hydroxyphenylacetic acid grafted poly- l-ysine hydrogel;
D O I
10.2147/JIR.S492465
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Purpose: Treatment of severe burn wound injury remains a significant clinical challenge as serious infections/complex repair process and irregulating inflammation response. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have a multidirectional differentiation potential and could repair multiple injuries under appropriate conditions. Poly(L-lysine)-graft-4-hydroxyphenylacetic acid (PLL-g-HPA) hydrogel is an enzyme-promoted biodegradable in hydrogel with good water absorption, biocompatibility and antibacterial properties. Therefore, the aim of this study was to evaluate the therapeutic effect of hUC-MSCs combined with PLL-g-HPA hydrogel on full thickness burn injury in rat model. Methods: The PLL-g-HPA hydrogel was developed and characterized by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), Hydrogen-1 nuclear magnetic resonance (H-NMR). The cytotoxicity to human foreskin fibroblasts (HFF) were assessed by CCK-8 assay and live/dead quantification and antibacterial activity against Escherichia coli and Staphylococcus aureus was also detected by colony forming unit. A full-thickness burn wound injury model in 12 SD rats was established, and the therapeutic effect of PLL-g-HPA hydrogel combined with hUC-MSCs was detected by healing time/Histology/ inflammation factor expression level. Results: The findings from SEM, FTIR, and HFF analyses demonstrated the successful synthesis of PLL-g-HPA hydrogels. These hydrogels exhibited low cytotoxicity at minimal concentrations while maintaining excellent moisture retention and antibacterial properties. Compared to the control group, treatment with PLL-g-HPA hydrogel in conjunction with hUC-MSCs significantly enhanced wound healing, modulated inflammatory responses, and promoted angiogenesis as well as re-epithelialization in rat models. Conclusion: The PLL-g-HPA hydrogel in conjunction with hUC-MSCs represents a promising therapeutic approach for the management of burn wounds.
引用
收藏
页码:779 / 789
页数:11
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