MicroRNAs in exhaled breath condensate: A pilot study of biomarker detection for lung cancer

被引：4
|
作者：
Rai, Divyanjali [1 ]
Pattnaik, Bijay [1 ]
Bangaru, Sunil [1 ]
Bhatraju, Naveen K. [2 ]
Tak, Jaya [1 ]
Kashyap, Seetu [1 ]
Verma, Umashankar [1 ]
Vadala, Rohit [1 ]
Yadav, Geetika [3 ]
Dhaliwal, R. S. [3 ]
Agrawal, Anurag [2 ]
Guleria, Randeep [1 ]
Mohan, Anant [1 ]
机构：
[1] All India Inst Med Sci, Dept Pulm Crit Care & Sleep Med, Breath Resp Dis Lab, New Delhi, India
[2] CSIR Inst Genom & Integrat Biol, Mol Immunogenet Lab, New Delhi, India
[3] Indian Council Med Res, New Delhi, India
来源：
CANCER TREATMENT AND RESEARCH COMMUNICATIONS | 2023年 / 35卷
关键词：
Lung cancer; miRNAs; Exhaled breath condensate; Biomarkers; EXPRESSION; INDIA;
D O I：
10.1016/j.ctarc.2023.100689
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Introduction: Quantitation of microRNAs secreted by lung cells can provide valuable information regarding lung health. Exhaled breath condensate (EBC) offers a non-invasive way to sample the secreted microRNAs, and could be used as diagnostic tools for lung cancer. Materials & Methods: EBC samples from twenty treatment-na & iuml;ve patients with pathologically confirmed lung cancer and twenty healthy subjects were profiled for miRNAs expression. Selected microRNAs were further validated, using quantitative-PCR, in an independent set of 10 subjects from both groups. Results: A total of 78 miRNAs were found to be significantly upregulated in the EBC of lung cancer patients compared to the control group. Six of these 78 miRNAs were shortlisted for validation. Of these, miR-31-3p, let7i, and miR-449c were significantly upregulated, exhibited good discriminatory power. Discussion: Differential expression of miRNAs secreted by lung cells could be quantitated in EBC samples, and could be used as a potential non-invasive tool for early diagnosis of lung cancer.
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页数：5
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