An In-vitro Evaluation of Tooth Discoloration and Shear Bond Strength of Glass Ionomer Cement Bonded to Tooth Surface Pretreated with Silver Diamine Fluoride and Glutathione Biomolecule

Background:Silver diamine fluoride (SDF) is employed in caries prevention and treatment; however, tooth discoloration post treatment is a significant disadvantage, which can be reduced using glutathione (GSH), a water soluble tripeptide.Aim:To evaluate and compare the effect of glutathione biomolecule (GSH) and potassium iodide (KI) along with SDF on tooth discoloration and shear bond strength of glass ionomer cement (GIC) on the tooth surface.Methods:Artificial caries were created on 48 extracted unblemished premolars and divided into four groups (SDF, SDF + KI, SDF + GSH, and Water). The solutions were applied according to the manufacturer's instructions, and the samples were incubated for 24 hours. The color assessment was recorded on days 1, 7, and 14 by using a spectrophotometer. Following the color assessment, all the treated samples were bonded with GIC. The shear bond strength was evaluated using a universal testing machine.Results:Mixing SDF with GSH initially reduced tooth discoloration. Although there was an increase in the discoloration after 2 weeks, it was still less when compared to the SDF group. The application of GSH and KI post SDF application had no significant difference in the shear bond strength of GIC on the tooth surface.Conclusions:The use of GSH along with SDF helps in reducing the discoloration without compromising the shear bond strength.