Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography

被引：0
|
作者：
De'Ath, Clare [1 ,2 ,3 ,9 ]
Oliva, Mizar F. [1 ,3 ,10 ]
Moulin, Martine [1 ,3 ]
Blakeley, Matthew P. [4 ]
Haertlein, Michael [1 ,3 ]
Mitchell, Edward P. [2 ,3 ]
Gavira, Jose A. [5 ]
Bowler, Matthew W. [6 ]
Forsyth, V. Trevor [7 ,8 ]
机构：
[1] Inst Laue Langevin, Life Sci Grp, 71 Ave Martyrs, F-38042 Grenoble, France
[2] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38042 Grenoble, France
[3] Partnership Struct Biol, 71 Ave Martyrs, F-38042 Grenoble, France
[4] Inst Laue Langevin, Large Scale Struct Grp, 71 Ave Martyrs, F-38042 Grenoble, France
[5] CSIC, Inst Andaluz Ciencias Tierra, Lab Estudios Cristalog, Ave Las Palmeras 4, Granada 18100, Spain
[6] European Mol Biol Lab, 71 Ave Martyrs, F-38042 Grenoble, France
[7] Lund Univ, Fac Med, SE-22184 Lund, Sweden
[8] LINXS Inst Adv Neutron & Xray Sci, Scheelevagen 19, SE-22370 Lund, Sweden
[9] Univ Oxford, Sir William Dunn Sch Pathol, S Parks Rd, Oxford OX1 3RE, England
[10] Katholieke Univ Leuven, Dept Microbiol Immunol & Transplantat, Rega Inst Med Res, Leuven, Belgium
来源：
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2025年 / 58卷
基金：
瑞典研究理事会;
关键词：
counter-diffusion crystallization; X-ray diffraction; neutron macromolecular crystallography; perdeuteration; large-volume crystals; transthyretin; crystallogenesis; macromolecular deuteration; amyloidogenesis; FULLY-AUTOMATIC CHARACTERIZATION; PROTEIN CRYSTALS; DATA-COLLECTION; MACROMOLECULAR CRYSTALLOGRAPHY; CAPILLARY COUNTERDIFFUSION; RESOLUTION NEUTRON; SAMPLE-EVALUATION; ROOM-TEMPERATURE; MASS-TRANSPORT; CRYSTALLIZATION;
D O I：
10.1107/S1600576724011191
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.
引用
收藏
页码：107 / 118
页数：12
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