Generation of a BEST1 Pr-EGFP reporter human embryonic stem cell line via CRISPR/Cas9 editing

被引：0
|
作者：
Lu, Shuaiyan [1 ,2 ,3 ]
Chen, Ming [1 ,3 ]
Liu, Xiaoyu [1 ,3 ]
Li, Jiahang [1 ,3 ]
Liu, Hui [1 ,2 ,3 ]
Li, Shasha [1 ,2 ,3 ]
机构：
[1] Wenzhou Med Univ, Eye Hosp, Natl Engn Res Ctr Ophthalmol & Optometry, Wenzhou, Peoples R China
[2] Zhejiang Lab Regenerat Med Vis & Brain Hlth, Oujiang Lab, Wenzhou, Peoples R China
[3] Wenzhou Med Univ, Eye Hosp, State Key Lab Ophthalmol Optometry & Visual Sci, Wenzhou, Peoples R China
来源：
STEM CELL RESEARCH | 2025年 / 82卷
基金：
中国国家自然科学基金;
关键词：
ESRG;
D O I：
10.1016/j.scr.2024.103625
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The retinal pigment epithelium (RPE) cell, located between the neural retina and choriocapillaris, is vital for retinal maintenance and photoreceptor function. Human embryonic stem cells (hESCs) provide a limitless source of RPE cells for transplantation. Using CRISPR/Cas9, we inserted a fusion of the BEST1 promoter (an RPEspecific marker) and the EGFP gene into the AAVS1 locus to track differentiation in hESC-induced RPE (hESC-iRPE). The resulting gene-edited line, WAe009-A-2 maintained a normal karyotype, expressed pluripotency markers, and demonstrated differentiation potential, making it invaluable for RPE development and therapeutic research.
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页数：6
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