ISOLATION AND IDENTIFICATION OF CELLULOSE DECOMPOSING BACTERIA FROM THE SOIL OF YUNNAN PROVINCE, CHINA

被引：0
|
作者：
Liu, Qiuyan [1 ,2 ,3 ]
Zhao, Dandan [1 ,4 ]
Zeng, Min [1 ,2 ,3 ]
Du, Juan [1 ,2 ,3 ]
Chen, Longwei [1 ,2 ,3 ]
Liu, Qing [1 ,2 ,3 ]
Liu, Guanghua [1 ,5 ]
Zhou, Xiao-Gang [1 ,2 ,3 ]
机构：
[1] Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650223, Yunnan, Peoples R China
[2] Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peoples R China
[3] Minist Agr, Key Lab Southwestern Crop Gene Resources & Germpla, Kunming 650023, Yunnan, Peoples R China
[4] Yunnan Acad Agr Sci, Inst Agr Econ & Informat, Kunming 650201, Yunnan, Peoples R China
[5] Yunnan Acad Agr Sci, Int Agr Res Inst, Kunming 650200, Yunnan, Peoples R China
来源：
BANGLADESH JOURNAL OF BOTANY | 2024年 / 53卷 / 03期
关键词：
Cellulose decomposition bacteria; Forage fermentation; Bacillus pumilus; CMC enzyme activity; Molecular identification;
D O I：
10.3329/bjb.v53i30.76602
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In this experiment sodium carboxymethyl cellulose (CMC-Na) was used as the only carbon source to isolate cellulose decomposing bacteria from the soil of Qiongzhusi Forest Park in Kunming, Yunnan Province. The ratio of hydrolytic circle diameter and colony diameter formed by the strain on cellulose Congo red medium was determined for primary screening, and then the filter paper disintegration test and CMC enzyme activity assay were used for rescreening. A cellulose decomposing bacterium (HS-2) with high enzyme activity was obtained, and its enzyme activity was up to 16.42 U/ml. (U means the unit of activity of enzymes) Gram stain and Spore stain reaction showed that HS-2 strain was Gram-positive Bacillus. The Bacillus strain was identified as Bacillus pumilus on the basis of physiological and biochemical tests, and 16S rDNA sequence analysis.
引用
收藏
页码：635 / 641
页数：7
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