Comparative transcriptomic analysis of goose astrovirus genotype 1 and 2 in goose embryonic fibroblasts

被引:1
|
作者
Ji, Jiashuang [1 ]
Ji, Longhai [1 ]
Dong, Xiaofeng [1 ]
Li, Wei [1 ]
Zhang, Wuchao [1 ]
Wang, Xiangqin [1 ]
Wang, Junli [1 ]
Lei, Baishi [1 ]
Wang, Zhenhua [3 ]
Yuan, Wanzhe [1 ,2 ]
Zhao, Kuan [1 ,2 ]
机构
[1] Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R China
[2] Hebei Agr Univ, Hebei Vet Biotechnol Innovat Ctr, Baoding, Peoples R China
[3] Hebei New Century Pharmaceut Co Ltd, Shijiazhuang, Peoples R China
关键词
gosling gout; GAstV-1; GAstV-2; virus isolation; transcriptomic; SEQUENCE;
D O I
10.1016/j.psj.2024.104347
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Gout in goslings has become widespread and caused huge economic losses for the goose industry. Emerging evidence suggests that goose astrovirus (GAstV) is a prominent etiological factor of gout in goslings. At present, 2 genotypes of GAstV have been identified named GAstV-1 and GAstV-2. Here, we isolated the GAstV-1 HBLY strain and GAstV-2 XT1 strain from HeBei province of China. The genome and proliferation characteristics of GAstV-1 and GAstV-2 were analyzed and the results showed that the whole genome identity was 53.8% to 55.8%, especially the nucleotide and amino acids identity of ORF2 and Cap protein was only 49.5% to 50.5% and 19.6% to 22.6 %. Interestingly, GAstV-1 and GAstV-2 with such low homology both can cause gout in goslings. To further explore this phenomenon, the whole genomic expression profile of goose embryonic fibroblasts (GEFs) infected with GAstV-1 was investigated in comparison with GAstV-2. The results revealed that 126 differentially expressed genes (DEGs) were identified between GAstV-1-infected and uninfected cells at 48 h postinfection (hpi), and 262 DEGs between GAstV-2 and uninfected. Among these, there are 15 commonly up-regulated genes and 19 commonly down-regulated genes. Gene ontology (GO) enrichment analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, and short time-series expression miner (STEM) analysis suggested that GAstV-1 can induce a higher innate immune response to GEFs, while GAstV-2 has a more pronounced effect on GEFs metabolic pathways. The transcriptomic analysis results significantly enhance our comprehension of the pathogenic mechanisms of GAstV.
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页数:14
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