Screening model in Caenorhabditis elegans for radioprotective natural products

PurposeIonizing radiation (IR) could induce damage such as DNA damage and oxidative stress. Natural products, like tea, have been demonstrated potential in mitigating these damages. However, the lack of efficient and rapid screening methods for natural products hinders their widespread application. To address this challenge, this study utilized Caenorhabditis elegans (C. elegans) as an in vivo model to investigate radioprotective natural products.MethodsL1 stage C. elegans were exposed to X-rays or 60Co gamma-rays at varying dosages (20, 50, and 100 Gy), then the growth, reproduction, and lifespan of the nematodes were observed. Different culture and sample-administered modes were tested. Known radioprotective agents, including Amifostine (WR2721), Lycium barbarum extract (LBE), and Trillium tschonoskii fraction (TTF), served as positive controls to validate the reliability of the model. The radioprotective activity of teas with different fermentation degrees was compared based on this screening model.ResultsA screening model in C. elegans was established by X-rays at 20 Gy. An appropriate sample-administrated approach was investigated, which involves adding the sample to the nematode growth medium (NGM) agar covered with inactivated Escherichia coli 2 h before irradiation. The known radioprotective agents (WR2721, LBE, and TTF) validated that the model is stable. Our results of the model application revealed that teas with lower fermentation levels, such as green tea and oolong tea, particularly the n-butanol and ethyl acetate fractions from oolong tea, exhibited significant radioprotective activity.ConclusionsThis study presents an effective in vivo approach for the initial screening of radioprotective natural products.