Development and characterization of starch/polyvinyl alcohol active films with slow-release property by utilizing Mucorracemosus Fresenius mycelium to load with clove essential oil

The controlled release active packaging film represents a novel technology that always can effectively slow down the release of active agents, extending their efficacy. Mucorracemosus Fresenius (MF) mycelium was prepared and used as an adsorption carrier to load clove essential oil (CEO). The CEO/MF complexes were incorporated into the starch/polyvinyl alcohol (Starch/PVA) matrix to develop active films. The effects of MF content on the films' properties were investigated. MF exhibited the internal hollow structure with diaphragm inside and showed antioxidant activity. The adsorption rate of MF on CEO was 238.09 %. As MF increased, the tensile strength, water contact angle and gas barrier properties (water vapor and oxygen) of the films containing CEO enhanced. The release rate of CEO from the films into food simulant (10 % ethanol) slowed down significantly with increasing of MF. Compared to the film without MF, the film with highest MF delayed 33 h to reach equilibrium. The films with different content of MF showed different antioxidant and antibacterial activities, and different preservation effects on shrimp. It showed a great prospect to develop controlled release active films by utilizing MF mycelium as an adsorption material, which enriched the technical solutions for developing controlled release active packaging films.