Protocol Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology

被引：0
|
作者：
Raveendran, Vineeth A. [1 ]
Pressey, Jessica C. [1 ]
Woodin, Melanie A. [1 ]
机构：
[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON M5S 3G5, Canada
来源：
STAR PROTOCOLS | 2025年 / 6卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
LIQUID-JUNCTION; COTRANSPORTER KCC2; EXCITATORY ACTIONS; CL-COTRANSPORTER; PATCH-CLAMP; GABA; MEMBRANE; HOMEOSTASIS; INHIBITION; DEFICITS;
D O I：
10.1016/j.xpro.2025.103628
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Inhibitory synaptic transmission mediated by the neurotransmitter g-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl-) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (EGABA). Here, we present a protocol to study the regulation of cation-chloride cotransporters and the strength of synaptic inhibition in cultured mouse cortical neurons using electrophysiology. We describe steps for culturing neurons isolated from postnatal pups and electrophysiological measurement of EGABA. For complete details on the use and execution of this protocol, please refer to Raveendran et al.1
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页数：22
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