A betaine-contained solution ameliorates cold ischemia reperfusion injury by suppressing ferroptosis in porcine kidney

被引:0
|
作者
Wang, Hao-Yu [1 ]
Wang, Yu [2 ]
Wu, Shaohua [3 ,4 ]
Ma, Jiangong [5 ]
Zou, Xunfeng [6 ,7 ]
机构
[1] Third Cent Hosp Tianjin, Hepatol & Gastroenterol, Tianjin 200192, Peoples R China
[2] Nankai Univ, Coll Chem, Dept Chem, Tianjin 300071, Peoples R China
[3] Nankai Univ, Coll Chem, Dept Chem, Tianjin 300071, Peoples R China
[4] Nankai Univ, Coll Chem, Key Lab Adv Energy Mat Chem MOE, Tianjin 300071, Peoples R China
[5] Tianjin First Cent Hosp, Sch Med, Clin Lab, Nankai, Peoples R China
[6] Nankai Univ, Tianjin Cent Hosp 1, Sch Med, Dept Gen Surg, Tianjin 300192, Peoples R China
[7] Nankai Univ, Tianjin Cent Hosp 1, Res Inst Transplant Med, Sch Med, Tianjin 300190, Peoples R China
来源
ADVANCES IN REDOX RESEARCH | 2025年 / 14卷
关键词
Renal ischemia/reperfusion; Ferroptosis; ACSL4; Porcine kidney; Normothermic mechanical perfusion; Organ preservation; ORGAN PRESERVATION; LIVER; ACSL4;
D O I
10.1016/j.arres.2025.100123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Intracellular iron-mediated lipid oxidative damage, referred to as ferroptosis, plays a crucial role in renal ischemia-reperfusion (I/R) injury. This study aims to investigate the effects of a betaine-contained organ preservation solution on renal ferroptosis using the normothermic machine perfusion (NMP) system. Methods: Healthy adult Bama miniature pigs were utilized in this study. Both kidneys were harvested and stored at 4 degrees C using three organ preservation solutions: Hypertonic Citrate Adenine (HCA) solution, University of Wisconsin (UW) solution, and modified multiple-saccharide (MS2) solution, for a duration of 24 h. The kidneys were subsequently divided into HCA, UW, and MS groups. The kidneys stored in cold for 2 h were used as a control. All kidneys were reperfused using the NMP system for 2 h. Biochemical indicators and ferroptosis-related markers, including iron levels, oxidative stress, and Acyl-CoA synthetase long-chain family member 4 (ACSL4), were measured. Results: During the 2-hour NMP, prolonged cold storage and reperfusion significantly induced ferroptosismediated renal I/R injury, resulting in severe renal tubular damage. Notably, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), ACSL4, and iron were significantly elevated in the HCA group compared to the control group (P < 0.05), while these levels were significantly reduced in the UW and MS groups. Additionally, the MS group exhibited results similar to those of the UW group in preventing renal I/R injury. Conclusion: This study indicates that the betaine-containing MS solution attenuates ferroptosis-mediated renal cold I/R injury by inhibiting oxidative stress.
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页数:9
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