Histone deacetylases synergistically regulate juvenile hormone signaling in the yellow fever mosquito, Aedes aegypti

被引:0
|
作者
Gaddelapati, Sharath Chandra [1 ,2 ]
Palli, Subba Reddy [1 ]
机构
[1] Univ Kentucky, Coll Agr Food & Environm, Dept Entomol, Lexington, KY 40546 USA
[2] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA
基金
美国国家卫生研究院;
关键词
Epigenetics; HDAC; Metamorphosis; JH; Kr-h1; KRUPPEL HOMOLOG 1; REPRESSION; COMPLEX;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Controlling Aedes aegypti mosquitoes is crucial for managing mosquito-transmitted diseases like dengue, zika, chikungunya, and yellow fever. One of the efficient methods to control mosquitoes is to block their progression from the larval to the adult stage. Juvenile hormones (JH) maintain the larval stage and ensure proper developmental timing for transitioning from larval-pupal-adult stages. Our previous studies showed that histone deacetylases (HDACs) regulate JH signaling and metamorphosis in the red flour beetle Tribolium castaneum. However, the role of HDACs in regulating JH signaling in Ae. aegypti mosquito is unknown. To investigate the role of HDACs in JH signaling, we knockdown each HDAC coding gene in Aag-2 cells derived from Ae. aegypti. Knockdown of HDAC1, HDAC4, and HDAC11 increased the expression of the JH primary response gene, Kr & uuml;ppel homolog 1 (Kr-h1), which represses the larval-pupal metamorphosis. Moreover, the simultaneous knockdown of these three HDACs synergistically increased the Kr-h1 promoter activity and its expression, mimicking JH action in inducing Kr-h1. Nevertheless, each HDAC regulates the transcription of different sets of genes, except for a few common genes involved in JH signaling. Furthermore, the knockdown of these HDACs in Ae. aegypti larvae caused different phenotypes apart from delayed pupation: HDAC1 knockdown caused larval growth retardation, body shrinkage, and eventual death; HDAC4 knockdown led to incomplete head capsule shedding after metamorphosis; and HDAC11 knockdown caused higher pupal mortality. Our data demonstrates functional overlap and distinct functions for HDAC1, HDAC4, and HDAC11 in modulating JH signaling, with each HDAC having a unique role in mosquito development.
引用
收藏
页数:9
相关论文
共 50 条
  • [21] Knockout of juvenile hormone receptor, Methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito, Aedes aegypti
    Zhu, Guan-Heng
    Jiao, Yaoyu
    Chereddy, Shankar C. R. R.
    Noh, Mi Young
    Palli, Subba Reddy
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (43) : 21501 - 21507
  • [22] EFFECTS OF METHOPRENE, A JUVENILE-HORMONE ANALOG, ON THE LARVAL AND PUPAL STAGES OF THE YELLOW-FEVER MOSQUITO, AEDES-AEGYPTI
    GORDON, R
    BURFORD, IR
    JOURNAL OF INSECT PHYSIOLOGY, 1984, 30 (04) : 279 - 286
  • [23] Juvenile hormone connects larval nutrition with target of rapamycin signaling in the mosquito Aedes aegypti
    Shiao, Shin-Hong
    Hansen, Immo A.
    Zhu, Jinsong
    Sieglaff, Douglas H.
    Raikhel, Alexander S.
    JOURNAL OF INSECT PHYSIOLOGY, 2008, 54 (01) : 231 - 239
  • [24] Ovary ecdysteroidogenic hormone functions independently of the insulin receptor in the yellow fever mosquito, Aedes aegypti
    Dhara, Animesh
    Eum, Jai-Hoon
    Robertson, Anne
    Gulia-Nuss, Monika
    Vogel, Kevin J.
    Clark, Kevin D.
    Graf, Rolf
    Brown, Mark R.
    Strand, Michael R.
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2013, 43 (12) : 1100 - 1108
  • [25] REGULATION OF JUVENILE-HORMONE LEVEL BY JUVENILE-HORMONE ESTERASE AFTER A BLOOD MEAL IN THE ADULT FEMALE YELLOW-FEVER MOSQUITO, AEDES-AEGYPTI
    SHAPIRO, AB
    HAGEDORN, HH
    AMERICAN ZOOLOGIST, 1985, 25 (04): : P74 - P74
  • [26] Identification of juvenile hormone-induced posttranslational modifications of methoprene tolerant and Kruppel homolog 1 in the yellow fever mosquito, Aedes aegypti
    Kim, Kyungbo
    Albishi, Najla M.
    Palli, Subba Reddy
    JOURNAL OF PROTEOMICS, 2021, 242
  • [27] CONDITIONS THAT LEAD TO POLYANDROUS BEHAVIOR IN THE YELLOW FEVER MOSQUITO AEDES AEGYPTI
    Degner, Ethan C.
    Harrington, Laura C.
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2015, 93 (04): : 552 - 552
  • [28] Automated measurement of oxygen consumption by the yellow fever mosquito, Aedes aegypti
    Diarra, GM
    Roberts, TW
    Christensen, BM
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 60 (05): : 859 - 864
  • [29] Neuropeptide F and its expression in the yellow fever mosquito, Aedes aegypti
    Stanek, DM
    Pohl, J
    Crim, JW
    Brown, MR
    PEPTIDES, 2002, 23 (08) : 1367 - 1378
  • [30] The effect of targeted silencing of ferritin in the yellow fever mosquito, Aedes aegypti
    Bhakta, Janki B.
    Geiser, Dawn L.
    Kohlhepp, Edwin A.
    Zhou, Guoli
    Winzerling, Joy J.
    FASEB JOURNAL, 2010, 24